Effect of dietary histamine on intestinal morphology, inflammatory status, and gut microbiota in yellow catfish (Pelteobagrus fulvidraco)

The toxic effect of dietary histamine on the intestine aquatic animals has been demonstrated, but reports morphological observation are limited. Thus, a feeding trial was conducted to determine intestinal histology, inflammatory status and gut microbiota yellow catfish (Pelteobagrus fulvidraco). Here, we showed that histamine-rich diets caused severe abnormality damage intestine, including decreased villi length reduced number. In addition, quantitative real-time PCR (qRT-PCR) demonstrates increased expression pro-inflammatory genes (Tnf?, Il1?, Il8) an anti-inflammatory gene (Il10). Furthermore, alpha-diversity (observed OTUs, Chao1, Shannon Simpson) beta-diversity (non-metric multidimensional scaling, with stress value 0.17) demonstrated alterations in composition diversity. Co-occurrence networks analysis community influenced number relationship between bacteria species phyla Acidobacteria, Proteobacteria, Bacteroidetes, which instability community. Additionally, random forest selected six bacterial as biomarkers separate three groups, Lachnospiraceae Blautia (V520), Bacteroidales S24.7 (V235), Chloroplast Streptophyta (V368), Actinomycetales Streptomycetaceae (V152), Clostridia Clostridiales (V491) Paraprevotellaceae Prevotella (V245). Finally, Pearson correlation V520, V235, V491 were negatively correlated factors positively factor (Il10), indicated might be anti-inflammatory. These findings improved our understanding histological damage, induction mucosa status, alteration microbiota.